Binning sequences using very sparse labels within a metagenome

Consensus Clustering for Binning Metagenome Sequences

The advances in next-generation sequencing technologies allow researchers to sequence in parallel millions of microbial organisms directly from environmental samples. The result of this “shotgun” sequencing are many short DNA fragments of different organisms, which constitute the basis for the field of metagenomics. Although there are big databases with known microbial DNA that allow us classif...

MetaBAT: Metagenome Binning based on Abundance and Tetranucleotide frequency

Improved metagenome assemblies and taxonomic binning using long-read circular consensus sequence data

DNA assembly is a core methodological step in metagenomic pipelines used to study the structure and function within microbial communities. Here we investigate the utility of Pacific Biosciences long and high accuracy circular consensus sequencing (CCS) reads for metagenomic projects. We compared the application and performance of both PacBio CCS and Illumina HiSeq data with assembly and taxonom...

Draft Genome of a Novel Chlorobi Member Assembled by Tetranucleotide Binning of a Hot Spring Metagenome

The genome of a member of the phylum Chlorobi was assembled from a shotgun metagenomic sequence of a hot spring in Mammoth Lakes, CA. This organism appears to be a novel, aerobic, photosynthetic Chlorobi member, expanding the knowledge of this underrepresented phylum.

Sparse Motion Segmentation using Propagation of Feature Labels

The paper considers the problem of extracting background and foreground motions from image sequences based on the estimated displacements of a small set of image blocks. As a novelty, the uncertainty of local motion estimates is analyzed and exploited in the fitting of parametric object motion models which is done within a competitive framework. Prediction of patch labels is based on the tempor...